Waters – LC Columns

MaxPeak Premier Columns

Stop Looking and Start Seeing

MaxPeak Premier Columns are designed to be the most universal column platform for chromatographers who need to reduce variability risks and save time, while increasing recovery and sensitivity. Non-specific adsorption (NSA) of metal sensitive compounds is an unpredictable challenge, leading to long column passivation times, chromatography with large RSDs, and broad peaks that can be a challenge to detect. MaxPeak Premier Columns feature MaxPeak High Performance Surfaces (HPS) which effectively reduce NSA losses due to metal interactions and provide:

• Reduced column passivation
• Increased reproducibility
• Enhanced sensitivity
• Improved peak shapes

UPLC Columns

More than 200 combinations of column configurations and chemistries, UPLC Columns combine faster separations with higher resolution and sensitivities by harnessing the full potential of small particles. All Waters UPLC Columns are designed, tested, and guaranteed for routine use in applications up to 18,000 psi (1241 bar).

UHPLC Columns

Our focused selection of UHPLC columns highlight the performance advantages modern UHPLC instrumentation. Limiting your column choices limits your ability to fully develop the potential of your analytical laboratory. Waters’ CORTECS and eXtended Performace (XP) Ultra-High Performance LC columns and sorbents provide capability that will surpass your current and future analytical challenges.

HPLC Columns

State-of-the-art Reversed-Phase and HILIC HPLC Columns

Waters offers a wide range of analytical and preparative chromatography columns to meet the needs of virtually every application. The Waters Column Advisor recommends the most appropriate column depending on the on the application requirements you need to consider and the column characteristics you are looking for. Separations developed on our analytical columns can be directly scaled up to preparative columns. We supply a wide range of phase chemistries in numerous column dimensions for preparative work to meet your scale-up needs. All chromatography columns meet stringent specifications to ensure excellent performance and column-to-column reproducibility. We also offer a wide range of accessoriesto support these columns.

ACQUITY APC Columns

Advanced Polymer Chromatography

ACQUITY APC™ Columns deliver advanced sorbent technology for separating complex synthetic polymer and macromolecular species. They deliver superior performance and reliability for high resolution polymer characterization compared to conventional GPC columns. ACQUITY APC Columns improve advanced polymer chromatography by:

• Improving Bed Stability. ACQUITY APC Columns eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching.
• Maximizing Efficiency. The optimized pore volumes and particle sizes provide unmatched separation speed, resolution and chromatographic efficiency.
• Providing Industry Leading Reproducibility. Each step of our column manufacturing process is tightly controlled to deliver exceptional product reliability.

Bioseparations Columns & Consumables

Innovating Solutions to Help Solve Your Toughest Challenges

Our scientists, engineers, and application chemists continue to develop innovative columns and sample preparation consumables that support HPLC, UPLC, UHPLC, LC-MS analyses or lab-scale purification of oligonucleotides, amino acids, peptides, proteins and glycans. Our promise is to provide you with:

• Solutions you can count on: Reproducible and robust columns and systems, QC tested the way you use it
• Results you can trust: Wide range of technical expertise/application notes, Data integrity with our informatics solutions
• Flexibility that you require: Scalability/Transferability (UPLC/UHPLC/HPLC), Easy-to-use automation

GPC & SEC Columns

For over 50 years, Waters has been the market leader in LC-based GPC and SEC analysis, providing the highest quality columns and applications support for both organic- and aqueous-based size separations. The names gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic technique that separates compounds based on their relative different sizes in solution. This isocratic-based separation is performed by injecting sample onto a carefully packed column(s) containing particles of defined pore size with the larger molecular weight species eluting before those compounds of smaller molecular weight (i.e., larger sized species elute prior to smaller sized compounds). Chromatographers with different backgrounds and application needs tend to refer to these same size-based separations by different terms. In general, the term GPC is used for those analyzing plastics or synthetic polymers (organic or water-soluble) using either organic or aqueous containing eluents. By comparison, SEC is more commonly used to describe size-based separations of biological compounds such as peptides and proteins.

Nano and Micro LC Columns

Columns designed for reliable nano, capillary, and microscale UPLC separations

Waters nano columns offer purposefully-designed hardware to meet the different demands of today’s nanoflow LC and LC-MS separations. The UPLC Column delivers superior chromatographic resolution and fully leverages the separation power of small sub-2-µm particles. The selected stationary phases for nano-LC and trapping columns provide an advantage developing fast and efficient separations for complex peptide and protein separations and other sample limited analyses.

• ACQUITY UPLC M-Class Columns (rated for 15,000 psi  [1,000 bar]) allow for faster separations and are compatible with the nanoACQUITY UPLC system.
• The iKey Separation Device is an enabling technology behind the ionKey/MS System. It eliminates the need for traditional fittings and greatly simplifies the microflow-LC user experience.

Preparative (Purification) HPLC Columns