BTX – Electrofusion systems
View details of the full range of BTX Electrofusion systems
Available Models
Technical Specifications
| Frequency | 1 MHz |
| Voltage | 0 – 75 Vrms |
| Duration | 0 – 99 sec |
| Post fusion AC | 1/10 of pre-fusion amplitude |
| Post fusion ramp | 1 – 9 sec |
| Pause between AC/DC | 50 µsec |
The Legacy ECM 2001 multi-purpose electro cell manipulation generator produces a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.
LEGACY ECM 2001 Generator
The LEGACY ECM 2001 is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a wide range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.
The Legacy ECM 2001 is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
Key Features
- Wide range of voltages from 5 to 3000 V
- Finer voltage discrimination
- Pulse durations from 10 µsec to 10 sec
- Arc quenching
- Digital display of actual voltage and pulse length delivered
Technical Specifications
| Voltage Range: LV Mode | 5 to 500 V in 1 V steps |
| Voltage Range: HV Mode | 505 to 3000 V in 1 V steps |
| Voltage Accuracy | 5% |
| Pulse Length Range: LV Mode | 10 to 999 µs in 1 µs steps |
| Pulse Length Range: HV Mode | 10 to 600 µs in 1 µs steps |
The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.
ECM 2001 Generator
The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
Waveforms
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.
Key Features
- AC waveform of 0.2 – 2.0 MHz
- Square wave electroporation capabilities
- A wide range of voltages from 5 V to 3000 V
- Fine voltage discrimination
- Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
- Capable of operating at low impedance loads
- Large touchscreen interface
Technical Specifications
| Pulse Function | Constant, linear, non-linear |
| Pulse Amplitude | 100 – 1000 V |
| Pulse Width Range | 20 – 1000 ms |
| AC Start Peak Range | 5 – 75 Vs |
| AC Stop Peak Range | 5 – 75 V |
| AC Frequency | 0.2 to 2.0 MHz |
| AC Duration | 0 to 60 s |
The Hybrimune Hybridoma Production System is an advanced electrofusion system for fast, efficient cell fusion in hybridoma production, hybrid cell formation, and dendritic-tumor cell fusions.
Yield Improvements of 10 Fold or More Compared to Standard PEG Fusion
The Hybrimune system includes an innovative fusion chamber design, proprietary BTXpress® Cytofusion medium and sophisticated, tri-phasic electric field pulses that quickly position cells and disrupt cell membranes for maximum cell fusion efficiency with short cycle-times and minimal heating or turbulence for excellent cell viability. In addition, Hybrimune delivers a cell alignment waveform which is a patented sequence that enhances hybridoma yield.
The Hybrimune system consists of a user-friendly, programmable waveform generator controlled through the User-Interface Application Software running on a Windows-based computer system (not included). The fusion chamber uses coaxial electrodes designed for optimal electric field stimulation, independent of bath height. In this way, pulse parameters defined with the low volume optimization chamber are directly applicable for the large volume production chamber. The optimization chamber includes a transparent bottom for microscope viewing during the process optimization. BTXpress Cytofusion medium is a specially formulated, low conductivity solution for robust cell fusion efficiency.
The Hybrimune boasts a gradual increase in AC amplitude to compress the cells for maximal cell-cell contact. The DC pulse is then applied. The researcher has the option of doing multiple pulses of different voltages and duration if required. A final AC waveform holds the cells in place and stabilizes the fusion as the force is gradually reduced. The waveform generator is fully-programmable for pulse parameter optimization to maximize efficiency and cell viability.
A computer is required for the application software but is not included in the system.
The Hybrimune is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
Brand
BTX has been at the forefront of electroporation technology since we introduced the first commercially available electroporator in 1983. For over 30 years, we have made it our priority to focus only on electroporation and electrofusion. This focus has allowed us to develop the experience and expertise to supply you with a broad selection of innovative in vivo and in vitro tools to advance your research.
Whatever your need, our range of electroporation systems and accessories covers virtually every electroporation and electrofusion application, including high throughput. Additionally BTX provides exceptional applications support and service before, during and after your purchase to ensure your success.