Quantabio – Sample Preparation
Quantabio extraction reagents provide a simplified and cost-effective alternative to traditional nucleic acid (NA) purification methods and are optimized to work in series with Quantabio ToughMix reagents.
Available Models
| Kit Size | Order Code |
|---|---|
| 10 rxn | Order (95213-010) |
| 50 rxn | Order (95213-050) |
Rapid extraction and purification of high-quality total DNA from cultured cells or tissue
Extracta Plus DNA kits offer rapid purification of total DNA from a variety of sample sources. Genomic, mitochondrial and pathogenic DNA can be collected from fresh/frozen animal cells, tissue, blood, and bacteria. The Extracta Plus Spin Column workflow enables simultaneous processing of multiple samples into purified DNA suitable for PCR, Southern blotting, RAPD, AFLP and next generation sequencing applications.
Key Features
- High yield – Obtain large quantities of high quality DNA, free from PCR inhibitors
- Versatility in sample types – Rapid purification of fresh or frozen tissue, cells, blood or bacteria
- Lab friendly – Silica based column eliminates toxic organic chemicals such as phenol/chloroform
- Downstream compatibility – Ideal for sensitive applications such as endpoint PCR, qPCR and next generation sequencing
Extracta Plus DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
| Kit Size | Order Code |
|---|---|
| 10 rxn | Order (95214-010) |
| 50 rxn | Order (95214-050) |
Rapid extraction and purification of high-quality total RNA from cultured cells or tissue
The Extracta Plus RNA kit efficiently extracts and purifies RNA from cells and tissues. Its spin column workflow delivers pure RNA in just 25 minutes, suitable for various applications. It includes DNA removal columns to eliminate genomic DNA contamination, making it ideal for sensitive applications like RT-qPCR and NGS.
Key Features
- Rapid Protocol – From cells or tissue samples to high-quality total RNA in just 25 mins
- Streamlined gDNA removal – Novel DNA removal column eliminates gDNA contamination and need for DNase treatment
- Extraction of low input quantities – Ability to extract RNA from as little as 10 cells
- Downstream compatibility – Ideal for applications such as RT-PCR, RT-qPCR and next generation sequencing
Extracta Plus RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
| Kit Size | Order Code |
|---|---|
| 1 x 10 mL | Order (95171-010) |
| 1 x 500 mL | Order (95171-500) |
PCR-ready genomic DNA from dried blood spots
Extracta DBS is a ready-to-use DNA extraction reagent for fast and efficient recovery of PCR-ready DNA from dried blood spots. The resulting DNA is free of PCR inhibitors and compatible with various PCR and sequencing methods, including TaqMan® hydrolysis probe real-time qPCR for precise blood DNA quantification.
Combine with ToughMix and maximize qPCR assay sensitivity
Extracta DBS is the perfect match with Quantabio ToughMix for sensitive and precise target quantification. The crude extraction combined with ToughMix, a Quantabio master mix that is tolerant to common PCR inhibitors, results in higher DNA yields independent of DNA sample inputs and qualities to enable accurate detection and high sensitivity even with low copy targets.
Reliable, Consistent Lot-to-Lot Performance
Low amounts and quality of DNA recovered from dried blood spots commonly restrict the utilization of DNA. To overcome these limitations, Extracta DBS increases the yield and quality allowing for efficient and reliable recovery of DNA from dried blood spots. Manufactured under stringent quality standards, Extracta DBS ensures uniform lot-to-lot performance resulting in reliable reproducibility in combination with Quantabio ToughMix.
Key Features
- Optimized for DNA extraction from dried blood spot punches
- Single reagent for PCR-ready DNA in 30 minutes
- Maximized assay sensitivity, lower Cq values, when combined with Quantabio ToughMix®
- Compatible with high-throughput automation for PCR, qPCR and NGS applications
Extracta DBS is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Brand
Quantabio is a leading provider of advanced DNA and RNA amplification reagents for the most demanding molecular testing applications in applied, translational, and life science research. Our team leverages decades of experience in developing pioneering amplification technologies to deliver cutting-edge products to researchers focused on critical cloning, PCR, qPCR, and Next-Generation Sequencing (NGS) applications. Based in Beverly, Massachusetts, Quantabio offers a growing portfolio of products through its international sales operations, as well as a global network of distributors and commercial service providers.