Quantabio – PCR Reagent
Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.
Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensure specific and efficient primer extension only after activation at 94°C. Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.
Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.
Available Models
| Kit Size | Order Code |
|---|---|
| 100 x 25 µL rxns (1 x 1.25 mL) | Order (95142-100) |
| 800 x 25 μL rxns (8 x 1.25 mL) | Order (95142-800) |
| 4000 x 25 μL rxns (1 x 50 mL) | Order (95142-04K) |
AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. The only user supplied components are DNA template and molecular grade water.
Key Features
- Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
- Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification
- Separate electrophoretic mobility dye reduces risk of post-PCR cross contamination with gel electrophoresis
AccuStart II PCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
| Kit Size | Order Code |
|---|---|
| 100 x 25 µL rxns (1 x 1.25 mL) | Order (95136-100) |
| 500 x 25 μL rxns (5 x 1.25 mL) | Order (95136-500) |
| 4000 x 25 μL rxns (1 x 50 mL) | Order (95136-04K) |
The repliQa HiFi ToughMix is a 2x, ready-to-use master mix with enhanced fidelity and rapid PCR results. It includes a genetically modified DNA polymerase and hot start antibodies, offering 90x higher fidelity than Taq. The fast extension times, ranging from 1-10 kb/sec, enable quick results, and its compatibility with various inhibitors makes it ideal for PCR, cloning, amplicon sequencing, and mutagenesis.
Key Features
- Contains premixed loading dye to streamline gel electrophoresis
- User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and withstands to repetitive freeze-thaw (≥ 20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart ensures sensitive and precise assay performance
| Kit Size | Order Code |
|---|---|
| 25 rxns | Order (95200-025) |
| 100 rxns | Order (95200-100) |
| 500 rxns | Order (95200-500) |
The repliQa HiFi ToughMix is a 2x, ready-to-use master mix with enhanced fidelity and rapid PCR results. It includes a genetically modified DNA polymerase and hot start antibodies, offering 90x higher fidelity than Taq. The fast extension times, ranging from 1-10 kb/sec, enable quick results, and its compatibility with various inhibitors makes it ideal for PCR, cloning, amplicon sequencing, and mutagenesis.
Key Features
- Contains premixed loading dye to streamline gel electrophoresis
- User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and withstands to repetitive freeze-thaw (≥ 20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart ensures sensitive and precise assay performance
| Kit Size | Order Code |
|---|---|
| 25 rxns | Order (95199-025) |
| 100 rxns | Order (95199-100) |
The AccuStart Long Range SuperMix is a 4x ready-to-use solution for high-fidelity, long-range DNA amplification. It utilizes two hot-start DNA polymerases, one with strong proofreading activity, and an optimized buffer for precise and efficient amplification of targets up to 24kb. It offers superior fidelity, broad GC-content compatibility, multiplex capabilities, and is suitable for diverse applications like endpoint PCR, sequencing, cloning, and HLA typing.
Key Features
- Amplify +24 kb gDNA and +40 kb lambda DNA
- 4x concentration enables superior sensitivity with low inputs (100 pg)
- Multiplex up to 6 targets with each target up to 6 kb in length
- Stabilized, single-tube SuperMix minimizes pipetting errors and hands-on-time
- Reliable lot-to-lot reproducibility ensures consistent results
- Amplification through uracils and primers containing inosines/uracils
AccuStart Long Range SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
| Kit Size | Order Code |
|---|---|
| 50 rxn | Order (95192-050) |
| 250 rxn | Order (95192-250) |
sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR mix designed for NGS library amplification prior to sequencing. It includes a primer mix for Illumina® sequencing adapters (P5 and P7 sequences). The hot-start, proofreading DNA polymerase enhances library amplification efficiency, reduces artifacts, and leads to higher yields and uniform coverage. This kit works with low DNA input (250 pg) and minimizes bias when amplifying AT- and GC-rich regions.
Key Features
- HiFi DNA polymerase engineered to minimize amplification bias
- Increased amplification efficiency resulting in higher yields
- Uniform coverage across challenging AT- and GC-rich regions
- Robust amplification from input DNA as low as 250 pg
- Cost-effective alternative to KAPA HiFi with improved performance
sparQ HiFi PCR Master Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Brand
Quantabio is a leading provider of advanced DNA and RNA amplification reagents for the most demanding molecular testing applications in applied, translational, and life science research. Our team leverages decades of experience in developing pioneering amplification technologies to deliver cutting-edge products to researchers focused on critical cloning, PCR, qPCR, and Next-Generation Sequencing (NGS) applications. Based in Beverly, Massachusetts, Quantabio offers a growing portfolio of products through its international sales operations, as well as a global network of distributors and commercial service providers.